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标题：Neuroprotective Effect of Oxyresveratrol from Smilacis Chinae Rhizome on Amyloid β Protein (25—35)-Induced Neurotoxicity in Cultured Rat Cortical Neurons
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作者：Ju Yeon Ban ; So-Young Jeon ; Thi Thuy Ha Nguyen 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：12
页码：2419-2424
DOI：10.1248/bpb.29.2419
出版社：The Pharmaceutical Society of Japan
摘要：We previously reported that Smilacis chinae rhizome inhibits amyloid β protein (25—35) (Aβ (25—35))-induced neurotoxicity in cultured rat cortical neurons. The present study evaluated the neuroprotective effect of oxyresveratrol isolated from Smilacis chinae rhizome against Aβ (25—35)-induced neurotoxicity on cultured rat cortical neurons. Oxyresveratrol over the concentration range of 1—10 μ M significantly inhibited 10 μ M Aβ (25—35)-induced neuronal cell death, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Oxyresveratrol (10 μ M ) inhibited 10 μ M Aβ (25—35)-induced elevation of cytosolic calcium concentration ([Ca²⁺]_c), which was measured by a fluorescent dye, Fluo-4 AM. Oxyresveratrol (1, 10 μ M ) also inhibited glutamate release into medium and reactive oxygen species (ROS) generation induced by 10 μ M Aβ (25—35). These results suggest that oxyresveratrol prevents Aβ (25—35)-induced neuronal cell damage by interfering with the increase of [Ca²⁺]_c, and then by inhibiting glutamate release and ROS generation. Furthermore, these effects of oxyresveratrol may be associated with the neuroprotective effect of Smilacis chinae rhizome.
关键词：oxyresveratrol;Smilax chinae rhizome;β-amyloid protein;neurotoxicity;cortical neuron;neuroprotection