文章基本信息

标题：Highly Acidic C-Terminal Domain of pp32 Is Required for the Interaction with Histone Chaperone, TAF-Ibeta
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作者：In-Seon Lee ; Sang-Min Oh ; Sung-Mi Kim 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：12
页码：2395-2398
DOI：10.1248/bpb.29.2395
出版社：The Pharmaceutical Society of Japan
摘要：We have previously reported that INHAT (inhibitor of acetyltransferases) complex subunits, TAF (template activating factor)-Ialpha, TAF-Ibeta and pp32 can inhibit histone acetylation and HAT (histone acetyltransferase)-dependent transcription by binding to histones. Evidences are accumulating that INHAT complex subunits have important regulatory roles in various cellular activities such as replication, transcription, and apoptosis etc . However, how these subunits interact each other remains largely unknown. Using immunoprecipitation (IP) and protein–protein interaction assays with TAF-Ibeta and pp32 deletion mutant proteins, we identify INHAT complex subunits, TAF-Ibeta and pp32 interaction requires highly acidic C-terminal domain of pp32. We also show that the interaction between the INHAT complex subunits is stronger in the presence of histones. In this study, we report that the synergistic inhibition of HAT-mediated transcription by TAF-Ibeta and pp32 is dependent on the highly acidic C-terminal domain of pp32.
关键词：inhibitor of acetyltransferases (INHAT);template activating factor (TAF)-Ibeta;pp32;transcription;protein interaction