文章基本信息

标题：Pigments from Uroleucon nigrotuberculatum Induce Apoptosis in HL60 Human Leukemia Cells, Implicating Intracellular Oxidative Stress and Activation of Caspases
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作者：Shinya Suzuki ; Mariko Tomita ; Masumi Hyodo 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：12
页码：2383-2387
DOI：10.1248/bpb.29.2383
出版社：The Pharmaceutical Society of Japan
摘要：Two pigmented compounds from Uroleucon nigrotuberculatum , rhododactynaphin- jc -1 (H427) and rhododactynaphin- jc -2 (H373), significantly diminished the cell viability of HL60 cells with IC₅₀ of 10 μ M and 30 μ M , respectively, in an 18 h-dye uptake assay. Both H427 and H373 augmented the levels of intracellular reactive oxygen species (ROS) and induced apoptosis as demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis. ROS augmentation by both H427 and H373 was inhibited by N -acetylcysteine (NAC) and α-tocopherol. The apoptosis induced by H427 was inhibited efficiently with NAC and caspase-8 inhibitor but less efficiently with α-tocopherol and caspase-9 inhibitor. These findings suggested that these pigments have pro-apoptotic activities via oxidative stress.
关键词：aphid;apoptosis;reactive oxygen species;caspase