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标题：Sensitive Determination of 4-(4-Bromophenyl)-4-hydroxypiperidine, a Metabolite of Bromperidol, in Rat Plasma by HPLC with Fluorescence Detection after Pre-column Derivatization Using 4-Fluoro-7-nitro-2,1,3-benzoxadiazole
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作者：Yasuhiko Higashi ; Shota Nakamura ; Youichi Fujii 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：12
页码：2479-2482
DOI：10.1248/bpb.29.2479
出版社：The Pharmaceutical Society of Japan
摘要：The purpose of this study was to determine the level of 4-(4-bromophenyl)-4-hydroxypiperidine (BPHP), a bromperidol (BRO) metabolite, in rat plasma by HPLC with fluorescence detection after pre-column derivatization using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). After basic extraction of the samples with benzene, derivatization with NBD-F was conducted in borate buffer (pH 8.0) at 60 °C for 3 min. Mexiletine was utilized through the procedure as an internal standard (IS). Retention times of the BPHP and IS derivatives were 7.7 and 11.5 min, respectively. The regression equation for BPHP showed good linearity in the range of 0.01—1 mg/ml with the detection limit of 0.003 μg/ml. The coefficient of variation was less than 12.0%. The recovery was satisfactory. This method was applied for a pharmacokinetic study of BPHP in comparison with 4-(4-chlorophenyl)-4-hydroxypiperidine (CPHP), the corresponding haloperidol (HAL) metabolite, in rats. The ratio of the area under the plasma concentration curve ( AUC ) after p.o. administration of BPHP to the AUC after i.p. administration of BPHP (46%) was lower than that of CPHP (56%), indicating that intestinal absorption of BPHP is lower than that of CPHP. The ratio of BRO metabolism to BPHP (48%) was 1.8-fold higher than that of HAL metabolism to CPHP (27%); the ratio was estimated as ( AUC_{p.o. ,A→B}/ AUC_{p.o. ,B})×100, where AUC_{p.o. ,A→B} is the AUC value of BPHP or CPHP after p.o. administration of BRO or HAL, and AUC_{p.o. ,B} is the AUC of BPHP or CPHP after administration of BPHP or CPHP, respectively. Our method provides a sensitive procedure for determination of BPHP in rat plasma and is suitable for pharmacokinetic studies of BPHP after BRO administration.
关键词：4-(4-bromophenyl)-4-hydroxypoperidine;bromperidol;derivatization;4-fluoro-7-nitro-2,1,3-benzoxadiazole;pharmacokinetic study