文章基本信息

标题：Accumulation of Mercury in Transgenic Tobacco Expressing Bacterial Polyphosphate
本地全文：下载
作者：Takeshi Nagata ; Chihiro Ishikawa ; Masako Kiyono 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：12
页码：2350-2353
DOI：10.1248/bpb.29.2350
出版社：The Pharmaceutical Society of Japan
摘要：The feasibility of transgenic tobacco, engineered to express bacterial polyphosphate (polyP), for phytoremediation of mercury pollution was evaluated. T₃ progeny of the transgenic tobacco produced a large amount of polyP in leaves and showed a relatively high resistant phenotype to Hg²⁺ than its wild-type progenitors. These results suggest that the integrated ppk gene, encoding polyphosphate kinase (PPK), a key enzyme for polyP biosynthesis, is stably conserved in tobacco genome, and translated to active PPK which catalyzed biosynthesis of polyP, and suggest that polyP is capable of reducing the cytotoxicity of Hg²⁺, probably via chelation formation with polyP. The transgenic tobacco expressing polyP accumulated significantly more mercury than its wild-type progenitors from Hg²⁺-containing agar medium and simulated soils without taxing the tobacco plants suggesting that the transported Hg²⁺ was accumulated as a less toxic Hg-polyP complex in the tobacco tissues. Based on the results obtained in the present study, the polyP-mediated accumulation of mercury from mercurial-contaminated soils may provide an ecologically compatible approach for phytoremediation of mercury pollution.
关键词：mercury accumulation;polyphosphate;transgenic tobacco;ppk;mercury resistance;phytoremediation