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标题：In Vitro Antiviral Activity of 1,2,3,4,6-Penta-O-galloyl-β-D-glucose against Hepatitis B Virus
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作者：Sung-Jin Lee ; Hak-Kyo Lee ; Min-Kyung Jung 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：10
页码：2131-2134
DOI：10.1248/bpb.29.2131
出版社：The Pharmaceutical Society of Japan
摘要：This study examined the antiviral activity of the root of Paeonia lactiflora P ALL . Among the solvent fractions of the crude drug, the ethyl acetate fraction showed anti-hepatitis B virus (HBV) activity (IC₅₀, 8.1 μg/ml) in an HBV-producing HepG2.2.15 cell culture system. The active anti-HBV principle was isolated and identified as 1,2,3,4,6-penta- O -galloyl-β- D -glucose (PGG) from the crude drug by activity-guided fractionation. PGG isolated from P. lactiflora was examined for the inhibition of HBV multiplication by measurement of HBV DNA and hepatitis B surface antigen (HBsAg) levels in the extracellular medium of HepG2.2.15 cells after 8-d treatment. PGG decreased the level of extracellular HBV (IC₅₀, 1.0 μg/ml) in a dose-dependent manner. PGG also reduced the HBsAg level by 25% at a concentration of 4 μg/ml. The gallate structure of PGG may play a critical role in the inhibition of anti-HBV activity. These results suggest that PGG could be a candidate for developing an anti-HBV agent.
关键词：Paeonia lactiflora;1,2,3,4,6-penta-O-galloyl-β- D -glucose;hepatitis B virus;hepatitis B surface antigen;HepG2.2.15 cell;anti-HBV activity