文章基本信息

标题：Neuroprotective Effect of Some Plant Extracts in Cultured CT105-Induced PC12 Cells
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作者：Sang Tae Kim ; Jeong Do Kim ; Yeoung-Su Lyu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：10
页码：2021-2024
DOI：10.1248/bpb.29.2021
出版社：The Pharmaceutical Society of Japan
摘要：Carboxyl-terminal fragments of APP (CT) have been found in plaques, microvessels and the neurofibrillary tangles in the brains of AD patients. These carboxyl-terminal fragments, which contain the complete Aβ sequence, appear to be toxic to neurons in culture cells. However, the possible role of other cleaved products of APP is less clear. We showed that a recombinant carboxy-terminal 105 amino acid fragment (CT105) of APP induced strong neurotoxicity in PC12 cells. We prepared alcoholic extract from Oriental herbal plants and screened their protective effects against CT₁₀₅-induced cell death in PC12 cells after the treatment of these extracts. Of the 10 kinds of plant extracts, 12 kinds of extracts had considerable protective effects against CT₁₀₅-induced cell death, especially, Uncariae Ramulus et Uncus (UREU), Gastrodia elata (GAE), Evodia officinalis (EO) and Panax ginseng (PAG) showed the most protective effect at the concentration of 50μg/ml. BuOH extract of UREU and GAE possessed the strongest protective effects against neurotoxicity of CT₁₀₅-induced PC12 cells and showed inhibitory effect with IC₅₀ values of 4.8 and 8.3μg/ml, respectively. These plants are promising candidates of neuroprotective effects and would be useful for the treatment of the neuronal degenerative diseases such as Alzheimer's diseases.
关键词：Alzheimer's disease;Uncariae Ramulus et Uncus;Gastrodia elata;beta-amyloid;neurodegeneration;CT105