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标题：Human Serum Albumin Enhances the Hemolytic Activity of Vibrio vulnificus
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作者：Mi-Hwa Choi ; Hui-Yu Sun ; Ra-Young Park 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2006
卷号：29
期号：1
页码：180-182
DOI：10.1248/bpb.29.180
出版社：The Pharmaceutical Society of Japan
摘要：Vibrio vulnificus hemolysin (VvhA) is inactivated in the late growth phase by its oligomerization. Albumin is known to affect the activities of many bacterial toxins. In this study, we investigated the effects of human or bovine serum albumin (HSA or BSA) on the production and activity of VvhA. HSA did not affect V. vulnificus growth and vvhA transcription. However, VvhA hemolytic activity in culture supernatants was significantly higher in the presence of HSA than in the absence of HSA. By Western blot analysis, the oligomerization of VvhA was inhibited and the remaining active VvhA monomer was increased in culture supernatants containing HSA. BSA produced similar results. These findings indicate that both HSA and BSA stabilize VvhA and delay VvhA inactivation by oligomerization, and thus enhance VvhA activity.
关键词：Vibrio vulnificus;hemolysin;human serum albumin;bovine serum albumin