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标题：Effect of Sodium Ferulate on Human Colonic Anion Secretion and the Underlying Signaling Mechanism
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作者：Wen Chao Zhao ; Jin Xia Zhu ; Gui Hong Zhang 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：9
页码：1608-1611
DOI：10.1248/bpb.28.1608
出版社：The Pharmaceutical Society of Japan
摘要：The present study investigated the effect of ferulic acid, a compound purified from traditional Chinese herbal medicine Chuanxiong and Awei , on anion secretion by human colonic cells (T₈₄) using the short circuit current ( I _SC) and microspectrofluorimetric technique. Basolateral administration of sodium ferulate (SF) produced a concentration-dependent increase of I _SC in T₈₄ cells with an EC₅₀ of 1.2 m M . The SF-induced increase in I _SC contained a transient peak followed by a sustained plateau. Removal of extracellular Cl⁻, basolateral addition of bumetanide, an inhibitor of the Na⁺–K⁺–Cl⁻ cotransporter (NKCC) and apical pretreatment with DPC, a Cl⁻ channels blocker, decreased the SF-induced increase in I _SC by 94% ( p <0.001), 84% ( p <0.001) and 85% ( p <0.001) respectively. Pretreatment with thapsigargin, a specific microsomal Ca²⁺-ATPase inhibitor, in combination with EGTA, a Ca²⁺ chelator, decreased the SF-induced peak by 52% ( p <0.01) and inhibited the SF-induced plateau by 60% ( p <0.05). Pretreatment with MDL12330A, an adenylate cyclase inhibitor, blocked the SF-induced I _SC plateau by 87% ( p <0.01) but did not affect the SF-induced I _SC peak. Microspectrofluorimetric measurements show that SF induced a sustained increase in [Ca²⁺]_i. The results suggested that SF could induce Cl⁻ secretion in T₈₄ cells via Ca²⁺ and cAMP-dependent pathways.
关键词：calcium; chloride; colonic cell; ferulic acid; sodium ferulate; T₈₄ cell