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标题：Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants
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作者：Yoshiki Mino ; Mayumi Yukita ; Nobuhiro Hiratsuka 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：8
页码：1535-1538
DOI：10.1248/bpb.28.1535
出版社：The Pharmaceutical Society of Japan
摘要：The complete amino acid sequences of [2Fe–2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S -carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1—8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants ( Scopolia japonica , Datura stramonium , D. metel , and D. arborea ), and only 1 or 4 differences compared to S. japonica and D. arborea . In contrast, 9—23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically.
关键词：Atropa belladonna;Hyoscyamus niger;Solanaceae;amino acid sequence;protein chemotaxonomy;ferredoxin