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标题：Effects of R-(−)-BPAP on the Expressions of Neurotrophins and Their Receptors in Mesencephalic Slices
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作者：Chieko Hirami ; Kazue Takahata ; Seiichiro Shimazu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：8
页码：1524-1526
DOI：10.1248/bpb.28.1524
出版社：The Pharmaceutical Society of Japan
摘要：R -(−)-1-(Benzofuran-2-yl)-2-propylaminopentane [ R -(−)-BPAP] enhances electric field stimulation-induced release of catecholamine from isolated brain stem and ameliorates motor deficits in rats. We evaluated the effects of R -(−)-BPAP on the expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), and their receptors, trkB and p75^NTR in rat mesencephalic slice cultures. Levels of mRNA and protein were measured at 48 h after R -(−)-BPAP treatment by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. R -(−)-BPAP significantly increased the mRNA and protein levels of BDNF, without affecting the level of NT-3 mRNA. In addition, R -(−)-BPAP significantly increased the mRNA level of trkB, but not that of p75^NTR. These effects of R -(−)-BPAP may result in enhanced BDNF/trkB signaling, and could thus underlie the potential neurotrophic and antidepressant actions of this drug.
关键词：R-(−)-1-(benzofuran-2-yl)-2-propylaminopentane;mesencephalon;brain-derived neurotrophic factor;trkB;slice culture;Parkinson's disease