文章基本信息

标题：Multidrug Resistance in Klebsiella pneumoniae MGH78578 and Cloning of Genes Responsible for the Resistance
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作者：Wakano Ogawa ; Dai-Wei Li ; Ping Yu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：8
页码：1505-1508
DOI：10.1248/bpb.28.1505
出版社：The Pharmaceutical Society of Japan
摘要：Klebsiella pneumoniae MGH78578, a clinical isolate, showed high level of resistance to many antimicrobial agents. We cloned genes responsible for drug resistance from chromosomal DNA of K. pneumoniae MGH78578 by shotgun method using Escherichia coli KAM32, a drug hypersensitive strain, as host. We obtained 43 hybrid plasmids that made host cells resistant to several antimicrobial agents. We classified them into 17 groups based on growth properties in the presence of each one of 9 antimicrobial agents and on restriction patterns of each hybrid plasmid. Analysis of the cloned genes must be very useful for investigation of major parts of multidrug resistance systems including multidrug efflux pumps in K. pneumoniae MGH78578 in which genome sequence is available.
关键词：multidrug resistance;multidrug efflux pump;gene cloning;Klebsiella pneumoniae