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标题：The Immunosuppressive Effect of Gamisanghyulyunbueum through Inhibition of Mitogen-Activated Protein Kinase and Nuclear Factor Activation in MOLT-4 Cells
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作者：Hye-Young Shin ; Hyun-Ja Jeong ; Ho-Jeong Na 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：7
页码：1177-1182
DOI：10.1248/bpb.28.1177
出版社：The Pharmaceutical Society of Japan
摘要：Gamisanghyulyunbueum (GSHYBE) has been used clinically to treat skin related disease in South Korea. We investigated GSHYBE-mediated changes in downstream T cell signal transduction. To determine the mechanism of inhibition, we have studied many of the major pathways in phytohemagglutinin (PHA)-activated T cell. We show that among the mitogen-activated protein kinase family activation of phosphorylation of extra cellular signal-regulated kinase 1/2 (ERK1/2, p44/42) and p38, but not c-jun NH₂-terminal kinase is inhibited. In activated MOLT-4 cells, the nuclear localization of nuclear factor of activated T cells (NFATc) was blocked by GSHYBE (1 mg/ml). Also, degradation of inhibitor κB-α and transactivation by nuclear factor-κB (NF-κB)/ Rel A were impaired by GSHYBE (1 mg/ml). Furthermore, interlukin (IL)-2, IL-4 and Interferen (IFN)-γ secretion by PHA activated MOLT-4 cells and peripheral blood mononuclear cells (PBMC) were significantly diminishes following GSHYBE treatment (1 mg/ml). Also, oral administration of GSHYBE inhibited IL-2 secretion in skin allergic reaction. In conclusion, our data indicate that GSHYBE treatment of T cells inhibits ERK1/2 and p38 activation and nuclear translocation of NFATc, NF-κB, resulting in diminished secretion of IL-2.
关键词：Gamisanghyulyunbueum;mitogen-activated protein kinase;NFATc;nuclear factor-κ/Rel A;interlukin-2