文章基本信息

标题：In Vitro Cytotoxicity of Stealth Liposomes Co-encapsulating Doxorubicin and Verapamil on Doxorubicin-Resistant Tumor Cells
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作者：JianCheng Wang ; BoonCher Goh ; WanLiang Lu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：5
页码：822-828
DOI：10.1248/bpb.28.822
出版社：The Pharmaceutical Society of Japan
摘要：Multidrug resistance (MDR) is a major obstacle to successful clinical cancer chemotherapy. A novel doxorubicin anti-resistant Stealth liposomes (DARSLs), prepared by co-encapsulating doxorubicin (DOX) and verapamil (VER) into stealth liposomes, has been developed. The average particle size of DARSLs was 118.1±22.3 nm. Encapsulation efficiencies of DOX and VER in DARSLs were greater than 95% and 70%, respectively. The IC₅₀ of DARSLs as measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) assay in multidrug resistant rat prostate cancer Mat-LyLu-B2 (MLLB2) cells was 0.079±0.017 μ M , 13 fold less than that for liposomal DOX with free VER (LDFV 0.96±0.46 μ M ) but only about 2 times less than FDFV. The IC₅₀ cytotoxicity on MLLB2 cells of the various formulations was as follows: DARSLs∼LDLV
关键词：cytotoxicity;multidrug resistance;stealth liposome;doxorubicin with verapamil