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标题：Neuroprotective and Antioxidant Effects of the Ethyl Acetate Fraction Prepared from Tussilago farfara L.
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作者：Jungsook Cho ; Hyang Mi Kim ; Jae-Ha Ryu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：3
页码：455-460
DOI：10.1248/bpb.28.455
出版社：The Pharmaceutical Society of Japan
摘要：The flower buds of Tussilago farfara L. (Compositae) have been traditionally used in Oriental medicine for the treatment of bronchitis and asthma. The extract of T. farfara was reported to exhibit antiinflammatory actions by inhibiting arachidonic acid metabolism and nitric oxide (NO) production in lipopolysaccharide-activated macrophages. In the present study, we investigated the effects of the ethyl acetate (EA) fraction on various types of neuronal cell damage induced in primary cultured rat cortical cells. Its antioxidant activities were also evaluated by cell-free bioassays. We found that the EA fraction potently inhibited the neuronal damage induced by arachidonic acid. We also found that it significantly attenuated the neuronal damage induced by spermine NONOate, a stable NO generator. In addition, it inhibited the A_β(25—35)-induced neurotoxicity and glutamate- or N -methyl- D -aspartic acid-induced excitotoxicity. It was found that the oxidative neuronal damage induced by H₂O₂, xanthine/xanthine oxidase, or Fe²⁺/ascorbic acid was also inhibited by the EA fraction. Furthermore, it was shown to inhibit lipid peroxidation initiated by Fe²⁺/ascorbic acid in rat brain homogenates, and scavenge DPPH radicals. This is the first demonstration of neuroprotective and antioxidant effects of T. farfara . Although complex mechanisms may be involved in the neuroprotective actions, T. farfara may be useful for the management of neurodegenerative disorders associated with inflammation, A_β, excitotoxicity, and/or oxidative stress.
关键词：Tussilago farfara;neuroprotection;antioxidant;cortical neurons;excitotoxicity;amyloid β (Aβ)