文章基本信息

标题：Degradation of Bisphenol A by White Rot Fungi, Stereum hirsutum and Heterobasidium insulare, and Reduction of Its Estrogenic Activity
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作者：Soo-Min Lee ; Bon-Wook Koo ; Joon-Weon Choi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：2
页码：201-207
DOI：10.1248/bpb.28.201
出版社：The Pharmaceutical Society of Japan
摘要：Two lignin-degrading basidiomycetes, Stereum hirsutum and Heterobasidium insulare , were used to degrade bisphenol A (BPA) in culture, and the estrogenic activity of the degradation products was examined using MCF-7 cell proliferation assays (E-screen) and analysis of pS2 mRNA expression in MCF 7 cells. Both S. hirsutum and H. insulare showed high resistance to BPA 100 ppm, and their mycelial growth was fully completed within 8 d of incubation at 30 °C. It took 7 to 14 d to achieve complete degradation ( ca. 99%) of BPA by both fungi. MCF-7 cells proliferated actively at a BPA concentration of 10⁻⁵ M . However, cell line proliferation was significantly inhibited when the cells were incubated in BPA culture media containing S. hirsutum and H. insulare . Similar results were obtained regarding pS2 mRNA expression. The pS2 mRNA expression levels decreased by 1.5-fold in supernatant from BPA treated with S. hirsutum and H. insulare compared with those treated with BPA alone.
关键词：bisphenol A;biodegradation;Stereum hirsutum;Heterobasidium insulare;estrogenic activity