文章基本信息

标题：Curcuma longa Extract Protects against Gastric Ulcers by Blocking H2 Histamine Receptors
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作者：Dong-Chan Kim ; Sun-Hee Kim ; Bo-Hwa Choi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：12
页码：2220-2224
DOI：10.1248/bpb.28.2220
出版社：The Pharmaceutical Society of Japan
摘要：Curcuma longa has been commonly used as a traditional remedy for a variety of symptoms such as inflammation, gastritis and gastric ulcer. When C. longa extract was administered per os to pylori-ligated rat stomachs, it reduced gastric acid secretion and protected against the formation of gastric mucosal lesions. We therefore tested whether C. longa extract inhibits gastric ulcers by blocking the H₂ histamine receptor. Dimaprit, a H₂ histamine receptor agonist, induced intracellular cAMP production in U937 and HL-60 promyelocytes. Pretreatment with C. longa extract significantly blocked dimaprit-induced cAMP production in a concentration dependent manner, but had no effect on the elevation of cAMP levels triggered by isoproterenol-induced β₂-adrenoceptor activation in U937 cells. To identify the active component(s) of C. longa extract, we sequentially fractionated it by extraction with ethyl acetate, n -butanol and water. We found that the ethyl acetate extract showed the most potent H₂R antagonistic effect against dimaprit-induced cAMP production. However, curcumin, a major component of C. longa extract, showed no H₂R blocking effect. C. longa ethanol extract and ethylacetate extract also blocked the binding of [³H]-tiotidine to membrane receptors on HL-60 cells. These findings suggest that the extract from C. longa specifically inhibits gastric acid secretion by blocking H₂ histamine receptors in a competitive manner.
关键词：Curcuma longa;H2 histamine receptor;gastric ulcer;gastric juice secretion