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标题：Involvement of Polyubiquitin Chains via Specific Chain Linkages in Stress Response in Mammalian Cells
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作者：Masahiro Fujimuro ; Tadashi Nishiya ; Yasuyuki Nomura 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：12
页码：2315-2318
DOI：10.1248/bpb.28.2315
出版社：The Pharmaceutical Society of Japan
摘要：Polyubiquitination plays key roles in various proteasome-dependent and independent cellular events. To elucidate roles in stress response of polyubiquitin chains formed via specific chain linkages in mammalian cells, we established NIH3T3 stable cell lines that are capable of conditionally expressing K29R, K48R and K63R ubiquitin mutants, in which the Lys29, Lys48 and Lys63 residues of ubiquitin had been changed to Arg, and we examined the effects of various stresses on their cell viabilities. The expression of K63R ubiquitin mutant decreased viability of the cells post-exposed to ethanol, H₂O₂ and methyl methanesulfonate (MMS), while that of K48R mutant decreased viability of the cells post-exposed to heat shock as well as ethanol, H₂O₂ and MMS. Thus, these results suggest that polyubiquitin chains formed via specific chain linkages are involved in the respective stress responses in mammalian cells.
关键词：ubiquitin;polyubiquitin chain;stress response;heat shock