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标题：Meso-dihydroguaiaretic Acid from Machilus thunbergii SIEB et ZUCC., and Its Effects on the Expression of Matrix Metalloproteinase-2, 9 Cause by Ultraviolet Irradiated Cultured Human Keratinocyte Cells (HaCaT)
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作者：Hyung In Moon ; Jin Ho Chung
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：11
页码：2176-2179
DOI：10.1248/bpb.28.2176
出版社：The Pharmaceutical Society of Japan
摘要：Ethanol and aqueous extracts of Machilus thunbergii used traditionally for the treatments a wide variety of diseases were screened in vitro for the matrix metalloproteinase (MMP)-9 inhibitor actions. Meso -dihydroguaiaretic acid from the stems bark of Machilus thunbergii showed significant MMP-9 inhibition in human keratinocyte cells cause by ultraviolet irradiation. Here we investigated the effect of meso -dihydroguaiaretic acid, which was isolated from Machilus thunbergii , on UV-induced premature skin aging. We studied the effect of meso -dihydroguaiaretic acid on UV-induced MMP-9 expression in an immortalized human keratinocyte cell line, HaCaT, in vitro . Acute UV irradiation induced MMP-9 expression at both the mRNA and protein levels and meso -dihydroguaiaretic acid suppressed this UV-induced MMP-9 expression in a dose-dependent manner. Taken together, these results show that meso -dihydroguaiaretic acid can prevent the harmful effects of UV that lead to skin aging. Therefore, we suggest that meso -dihydroguaiaretic acid should be viewed as a potential therapeutic agent for preventing and/or treating premature skin aging.
关键词：Machilus thunbergii;meso-dihydroguaiaretic acid;ultraviolet irradiation;matrix metalloproteinase (MMP)-9