文章基本信息

标题：Effect of Solution Composition of Plasmid DNA on Gene Transfection Following Liver Surface Administration in Mice
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作者：Ryu Hirayama ; Shintaro Fumoto ; Koyo Nishida 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2005
卷号：28
期号：11
页码：2166-2169
DOI：10.1248/bpb.28.2166
出版社：The Pharmaceutical Society of Japan
摘要：We investigated the effect of plasmid DNA (pDNA) solution composition on gene transfection following liver surface administration in mice. Gene transfection experiments in situ and in vivo were performed using the following pDNA solutions: dextrose solution, NaCl solution, phosphate buffer, phosphate-buffered saline, Tris/HCl buffer with EDTA, Tris/HCl buffer with EDTA and Triton X-100, and water. In in situ experiments, we used a glass cylindrical diffusion cell that limited the contact area between the liver surface and the naked pDNA solution. The gene transfection at the site of diffusion cell attachment increased in hypotonic solution, and decreased in hypertonic solution, compared with isotonic solution. In in vivo experiments, instillation of naked pDNA solution onto the liver surface using a micropipette caused no significant differences in gene transfection in the applied lobe. These results suggest that it is important to select the optimal pDNA solution composition to control the gene transfection.
关键词：gene therapy;solution composition;osmotic pressure;plasmid DNA;liver;mouse