文章基本信息

标题：Methylene Chloride Fraction of Spatholobi Caulis Induces Apoptosis via Caspase Dependent Pathway in U937 Cells
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作者：Eun-Suk Ha ; Eun-Ok Lee ; Taek-Joon Yoon 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：9
页码：1348-1352
DOI：10.1248/bpb.27.1348
出版社：The Pharmaceutical Society of Japan
摘要：Spatholobi Caulis has been used in Oriental medicine to treat cancer and blood stasis. In this study, the methylene chloride fraction of Spatholobi Caulis (MCSC) was examined to determine if it possesses anti-cancer activity via its apoptosis-inducing activity. MCSC exhibited a strong cytotoxic effect against human monocyte leukemia U937 cells (IC₅₀=15.1 μg/ml). A TUNEL assay showed that the MCSC caused a characteristic ladder pattern of discontinuous DNA fragments and apoptotic bodies. Flow cytometric analysis confirmed that MCSC significantly increases the number of apoptotic cells stained by annexin V⁺/PI⁻ cells. Western blotting revealed that MCSC activated caspase-3 expression and cleaved poly (ADP-ribose) polymerase (PARP) in a concentration-dependent manner. An enzyme-linked immunosorbent assay (ELISA) demonstrated that MCSC significantly activated the caspase-3 activity compared with the untreated control by. Taken together, these results suggest that MCSC can induce apoptosis in U937cells via the caspase dependent pathway.
关键词：Spatholobi Caulis;U937;apoptosis;annexin V;caspase-3