文章基本信息

标题：Effect of Astilbe koreana on Ultraviolet B (UVB)-Induced Inflammatory Response in Human Keratinocytes
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作者：MinKyun Na ; Byung Sun Min ; Ren Bo An 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：8
页码：1301-1304
DOI：10.1248/bpb.27.1301
出版社：The Pharmaceutical Society of Japan
摘要：The EtOH extract from the rhizomes of Astilbe koreana (Saxifragaceae) exhibited potent antioxidant activity in our recent study. Since the oxidative stress is known to be involved in the inflammatory response after ultraviolet B (UVB) exposure, the ability of the A. koreana extract to inhibit UVB-induced prostaglandin E₂ (PGE₂) and nitric oxide (NO) production was examined. UVB irradiation (35 mJ/cm²) increased PGE₂ and NO production, which were significantly decreased by pre-administration of the A. koreana extract in a dose-dependent manner. The A. koreana extract also preserved cellular antioxidant capacity after UVB irradiation, which was determined by glutathione (GSH) content. UVB irradiation enhanced the formation of ROS in the keratinocytes, which was determined using a 2,7′-dichlorofluorescein diacetate (DCFH-DA), a redox sensitive dye. The levels of intracellular reactive oxygen species (ROS) were also significantly reduced by pre-treatment of the A. koreana extract in a dose-dependent manner measured 9 h after UVB irradiation. The results suggest that the A. koreana extract may have a protective effect on the UVB-injured keratinocytes by inhibiting PGE₂ and NO production, possibly through the inhibition of intracellular ROS accumulation.
关键词：Astilbe koreana;Saxifragaceae;ultraviolet B (UVB)-induced prostaglandin E2 (PGE2) production;UVB-induced nitric oxide (NO) production;intracellular reactive oxygen species (ROS)