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标题：The Combination of Rat Mast Cell and Rabbit Aortic Smooth Muscle Is the Simple Bioassay for the Screening of Anti-allergic Ingreditent from Methanolic Extract of Corydalis Tuber
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作者：Shin-ya Saito ; Masashi Tanaka ; Kimihiro Matsunaga 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：8
页码：1270-1274
DOI：10.1248/bpb.27.1270
出版社：The Pharmaceutical Society of Japan
摘要：We have assessed the release of histamine from mast cells by smooth muscle contraction. 0.3 μg/ml compound 48/80 showed no effect on concentration–response relationship of histamine in rabbit aorta. Compound 48/80 induced release of histamine from rat mast cells. When aorta was stimulated by compound 48/80 in the presence of mast cells, contraction was evoked in concentration-dependent manner. This mast cell-dependent contraction was completely blocked by H₁ receptor antagonist, 1 μ M diphenhydramine. When mast cells was treated with compound 48/80 inhibitor benzalkonium chloride, mast cell-dependent contraction was inhibited, although benzalkonium chloride itself showed no effect on concentration–response relationship of histamine in rabbit aorta. At high concentration of 10 μg/ml, benzalkonium chloride itself evoked histamine release from mast cells and indeed inhibitory effect of 10 μg/ml benzalkonium chloride on mast cell-dependent contraction was lower than that of 3 μg/ml. We have applied this bioassay to search anti-allergic ingredient from a total methanolic extract of Corydalis tuber ( Corydalis turtschaninovii B ESSER forma yanhusuo Y. H. C HOU et C. C. H SU ). Successively, we have isolated five fractions. The fractions I—IV are identified to be corybulbine (1), tetrahydropalmatine (2), corydaline (3) and yuanhunine (4), respectively. Main component of fraction V is the mixture of 3 and canadine (5). Fractions II and V significantly inhibited mast cell-dependent contraction in rabbit aorta as well as inhibited histamine release from rat mast cells. Furthermore, fractions I, III and V inhibited histamine-induced contraction in rabbit aorta at non-competitive manner. From these results, combination of rat mast cells and rabbit aorta is good bioassay to search the anti-allergic ingredient, and we have obtained effective fractions from Corydalis tuber using this assay.
关键词：Corydalis tuber;rat mast cell;rabbit aorta;anti-allergic ingredient