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标题：The Feeding of β-Carotene Down-Regulates Serum IgE Levels and Inhibits the Type I Allergic Response in Mice
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作者：Yuji Sato ; Hiroshi Akiyama ; Hiroyuki Suganuma 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：7
页码：978-984
DOI：10.1248/bpb.27.978
出版社：The Pharmaceutical Society of Japan
摘要：Feed containing β-carotene was administered orally to BALB/c mice immunized intraperitoneally with ovalbumin (OVA) for approximately 1 month. The titers of OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a in the mouse sera were determined. The OVA-specific IgE titer and OVA-specific IgG1 titer by mice fed β-carotene were significantly inhibited. On the other hand, the OVA-specific IgG2a titer in mice fed β-carotene was significantly greater than those of control mice. The OVA-specific IgE suppression of β-carotene feeding was dose-dependent. We also examined the effect of fed β-carotene on active systemic anaphylaxis. Feeding β-carotene to mice immunized with OVA inhibited the immediate reduction of the body temperature induced by antigen stimulation. Furthermore, the increase in serum histamine in the mice fed β-carotene under active systemic anaphylaxis was lower than in controls. We then examined the pattern of cytokine production by spleen cells from mice followed by restimulation with OVA in vitro . The spleen cells from the mice fed β-carotene produced more IFN-γ, IL-12 and IL-2 than those from the control group. In contrast, the spleen cells from the mice fed β-carotene produced less IL-4, IL-5, IL-6, IL-10 than those from the control group. Furthermore, analysis of IFN-γ mRNA levels of the splenocytes using the real-time quantitative RT-PCR technique revealed higher levels in the splenocytes from the mice fed β-carotene. These findings suggest that feeding β-carotene improves the helper T cell (T_H)1–T_H2 balance, inhibiting specific IgE and IgG1 production and antigen-induced anaphylactic response.
关键词：β-carotene;IgE;cytokine;TH1–TH2 balance;active systemic anaphylaxis