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标题：Increased in Vitro Release of Interferon-Gamma from Ampicillin-Stimulated Peripheral Blood Mononuclear Cells in Stevens–Johnson Syndrome
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作者：Tomoaki Yoshimura ; Emi Hamaguchi ; Eiseki Usami 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：6
页码：929-931
DOI：10.1248/bpb.27.929
出版社：The Pharmaceutical Society of Japan
摘要：Stevens–Johnson syndrome (SJS) was diagnosed in a 39-year-old woman, treated with ampicillin (4000—8000 mg daily), phenytoin (250 mg daily), and furosemide (20—40 mg daily) for 25, 21, and 20 d, respectively, before the appearance of the eruption. The lymphocyte stimulation test with the MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay on ampicillin, phenytoin, and furosemide showed a low value of stimulation index, which indicated negative reactivity. An in vitro IFN-γ release test was conducted on the patient with SJS and on two healthy controls. IFN-γ release increased by 52% following the in vitro challenge of the patient's peripheral blood mononuclear cells (PBMCs) with 15 μg/ml of ampicillin, but not with phenytoin or furosemide. Neither of the controls experienced increased IFN-γ release. In the present case, increased in vitro IFN-γ release was observed on ampicillin-stimulated PBMCs, which may indicate the role of ampicillin as the drug responsible for the induction of SJS, and may imply the role of IFN-γ in the pathogenesis of SJS.
关键词：Stevens–Johnson syndrome;interferon-γ;ampicillin;in vitro