文章基本信息

标题：Induction of Transition and Transversion Mutations during Random Mutagenesis PCR by the Addition of 2-Hydroxy-dATP
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作者：Hiroyuki Kamiya ; Mana Ito ; Hideyoshi Harashima 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：5
页码：621-623
DOI：10.1248/bpb.27.621
出版社：The Pharmaceutical Society of Japan
摘要：A 2-substituted purine nucleotide analog, 2-hydroxy-2′-deoxyadenosine 5′-triphosphate (2-OH-dATP), was added to a PCR mixture, to examine its mutagenic potential. The 2-OH-dATP enhanced the total mutation frequency. Interestingly, 2-OH-dATP induced both transition and transversion mutations, including A:T→G:C, A:T→C:G and G:C→T:A mutations. In contrast, other 2-substituted purine nucleotide analogs, 2-aminopurine-2′-deoxyriboside 5′-triphosphate and 2-amino-2′-deoxyadenosine 5′-triphosphate, did not affect the total mutation frequency. These results suggest that 2-OH-dATP is useful in random PCR mutagenesis for the in vitro evolution of nucleic acids and proteins, and for analyses of residues in these biomolecules.
关键词：mutagenesis PCR;2-hydroxy-dATP;transition;transversion