文章基本信息

标题：Estrogenic and Antiestrogenic Activities of the Roots of Moghania philippinensis and Their Constituents
本地全文：下载
作者：Eun-Mi Ahn ; Norio Nakamura ; Teruaki Akao 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：4
页码：548-553
DOI：10.1248/bpb.27.548
出版社：The Pharmaceutical Society of Japan
摘要：In the course of our search for natural estrogenic compounds from medicinal plants, we found that the methanolic extract from the roots of Moghania philippinensis (Fabaceae) showed significant effects on the proliferation of MCF-7 cells (human breast cancer) and induction of β-galactosidase activity in a yeast two-hybrid assay. Through estrogenic activity-guided fractionation, we isolated several active flavonoids including prenylated ones. The CHCl₃ fraction and its new constituent, 8-(1,1-dimethylallyl)genistein (9), appreciably increased the uterine weight in ovariectomized rats when administered orally for 14 consecutive days, in which compound 9 showed stronger estrogenic activity than genistein. Antiestrogenic activities were also examined based on the inhibition of MCF-7 cell proliferation and β-galactosidase activity in the yeast two-hybrid assay, mediated by 17β-estradiol. 5,7,3′,4′-Tetrahydroxy-6,8-diprenylisoflavone (6) showed the strongest antiestrogenic activity.
关键词：Moghania philippinensis;MCF-7;yeast two-hybrid assay;8-(1,1-dimethylallyl)genistein;phytoestrogen