文章基本信息

标题：Oxidative Stress Induces GSTP1 and CYP3A4 Expression in the Human Erythroleukemia Cell Line, K562
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作者：Fusako Nagai ; Eriko Kato ; Hiro-omi Tamura 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：4
页码：492-495
DOI：10.1248/bpb.27.492
出版社：The Pharmaceutical Society of Japan
摘要：To explore the effects of oxidative stress on metabolizing enzymes in blood cells, we examined the effect of hydrogen peroxide (H₂O₂) on glutathione S -transferase (GST) and cytochrome P450 (CYP) expression in a human erythroleukemic cell line, K562. After adding H₂O₂ (up to 100 μ M ) to the culture medium of K562 cells, the expression levels of GST and CYP enzymes were monitored by RT-PCR. The expression of GSTP1 and CYP3A4 was induced by oxidative stress. Quantitative PCR and immunoblot analysis revealed a 3- to 4-fold increase in GSTP1 and CYP3A4 mRNA, and a 2- to 3-fold increase in GSTP1 and CYP3A4 protein levels, 3 d after the addition of 100 μ M H₂O₂. Induction was H₂O₂ dose-dependent and also depended on the length of culture. Our results suggest that oxidative stress may affect GST and/or CYP expression in human blood cells, which may alter the metabolism of drugs and xenobiotics and thus, the toxicity of these compounds to the blood cells.
关键词：oxidative stress;K562;GSTP1;CYP3A4;induction