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标题：Effect of Herbal Medicines on Cytokine-Induced Cytotoxicity and Major Histocompatibility Complex (MHC) Class II Antigen Expression in Rat Thyroid Cells
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作者：Yun-Hee Shon ; Hee-Soon Lee ; Cheorl-Ho Kim 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：3
页码：371-374
DOI：10.1248/bpb.27.371
出版社：The Pharmaceutical Society of Japan
摘要：The effects of herbal medicines that constitute Gam-du-tang and Gung-gui-tang on cytokine-induced cytotoxicity and thyroid major histocompatibility complex (MHC) class II antigen expression in FRTL rat thyrocytes were investigated. No effect on cell growth was found with interferon (IFN)-γ. However, tumor necrosis factor (TNF)-α was cytotoxic, and this was increased by preincubation with IFN-γ. Ethanol extract of Glycyrrhizae Radix, black beans, Angelicae Radix, and Cnidii Rhizoma (0.3—15 mg/ml) in both regimens significantly inhibited IFN-γ and TNF-α-mediated cytotoxicity of rat thyroid cells ( p <0.05, p <0.01). In addition, IFN-γ (1—100 U/ml) treatment induced class II antigen expression in up to 60% of FRTL cells, as detected by a murine monoclonal antibody to rat MHC class II antigen (FITC-OX6). Aberrant thyroid cell MHC class II antigen expression induced by IFN-γ is suppressed by the extract of herbal medicines. These results indicate that herbal medicines inhibit cytokine-induced thyroid cell destruction, therefore, may have therapeutic potential in the treatment of autoimmune thyroid disease.
关键词：herbal medicine;autoimmune thyroid disease;interferon-γ;tumor necrosis factor-α;major histocompatibility complex class II antigen