文章基本信息

标题：Oral Administration of β-Cryptoxanthin Induces Anabolic Effects on Bone Components in the Femoral Tissues of Rats in Vivo
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作者：Satoshi Uchiyama ; Takashi Sumida ; Masayoshi Yamaguchi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：2
页码：232-235
DOI：10.1248/bpb.27.232
出版社：The Pharmaceutical Society of Japan
摘要：The effect of β-cryptoxanthin on bone components in the femoral tissues of rats was investigated. β-Cryptoxanthin was isolated from Satsuma mandarin ( Citrus unshiu M ARC .). Bone tissues were cultured for 48 h in serum-free Dulbecco's modified Eagle's medium containing either vehicle or β-cryptoxanthin (10⁻⁷ or 10⁻⁶ M ). The presence of β-cryptoxanthin (10⁻⁷ or 10⁻⁶ M ) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues. These increases were completely abolished in the presence of cycloheximide (10⁻⁶ M ), an inhibitor of protein synthesis. Thus β-cryptoxanthin had an anabolic effect on bone calcificantion in vitro . Moreover, β-cryptoxanthin (10, 25, or 50 μg/100 g body weight) was orally administered once daily for 7 d to young male rats. The administration of β-cryptoxanthin (10, 25, or 50 μg/100 g body weight) caused a significant increase in calcium content and alkaline phosphatase activity in the femoral-diaphyseal and femoral-metaphyseal tissues. Femoral-diaphyseal and femoral-metaphyseal DNA contents were significantly increased by the dose of 25 or 50 μg/100 g body weight. A significant increase in metaphyseal DNA content was also seen with the dose of 10 μg/100 g body weight of β-cryptoxanthin. This study demonstrates that β-cryptoxanthin has an anabolic effect on bone components in rats in vitro and in vivo .
关键词：β-cryptoxanthin;bone formation;bone metabolism;rat femur