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标题：Aminopeptidase N/CD13 Regulates the Fetal Liver Microenvironment of Hematopoiesis
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作者：Naoki Sakane ; Yoko Asano ; Tomoko Kawamura 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：12
页码：2014-2020
DOI：10.1248/bpb.27.2014
出版社：The Pharmaceutical Society of Japan
摘要：Fetal liver (FL) hematopoiesis is thought to be important for expanding the cell number during ontogeny. In order to investigate the cellular interaction molecules among FL stromal and hematopoietic cells, we established a monoclonal antibody, Ndk-10, that reacts with FL stromal cells but not with dish non-adherent cells. When Ndk-10 was added to an FL stromal and hematopoietic cell-coculture, it inhibited the survival of c-kit⁺ cells. The inhibitory activity of Ndk-10 was also observed in the fetal liver organ culture. The Ndk-10 recognized a 150 kD molecule in the adherent cells of FL and kidney, and the N-terminal amino acid sequence was identical to that of mouse aminopeptidase N/CD13. The peptidase activity of CD13 was inhibited by Ndk-10, and addition of its specific inhibitor resulted in the same inhibitory activity as Ndk-10. We propose that aminopeptidase N/CD13 is a critical molecule that regulates the survival of c-kit⁺ cells in the FL microenvironment.
关键词：fetal liver;microenvironment;CD13;aminopeptidase;hematopoiesis;stromal cell