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标题：cDNA Isolation and Functional Expression of Myrcene Synthase from Perilla frutescens
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作者：Madoka Hosoi ; Michiho Ito ; Toru Yagura 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：12
页码：1979-1985
DOI：10.1248/bpb.27.1979
出版社：The Pharmaceutical Society of Japan
摘要：cDNA cloning of a monoterpene synthase from Perilla frutescens whose steam-distilled oil contains 92.9% perillaketone, was performed by the PCR method using primers designed based on limonene synthase. The full-length nucleotide sequence of this cDNA consisted of 1978 bp including a 1827-bp translational region encoding a deduced protein of 608 amino acids, which was similar to that of limonene synthase from P. frutescens (85% identity). Functional expression of this clone in Escherichia coli yielded an active monoterpene synthase enzyme, which converted geranyl diphosphate into 53.8% myrcene, 20.9% sabinene, 19.8% linalool and 5.5% limonene. As for the extraction of reaction products, we performed SPME (solid phase micro extraction) as well as conventional solvent extraction, and compared these two extraction methods.
关键词：Perilla frutescens;myrcene synthase;homology-based cDNA cloning;Labiatae;monoterpene synthase;essential oil