文章基本信息

标题：Limitation of Polymyxin B on Suppression of Endotoxin Shock Induced by Salmonella Infection in Mice
本地全文：下载
作者：Hiroyuki Morita ; Ryoichi Hasunuma ; Kiichiro Kawaguchi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：11
页码：1840-1843
DOI：10.1248/bpb.27.1840
出版社：The Pharmaceutical Society of Japan
摘要：The protective effects of an antibiotic polymyxin B (PLB), having lipopolysaccharide (LPS)-binding activity, on infection-induced endotoxin shock in mice were investigated. Infection with 10⁸ colony forming units of an attenuated Salmonella typhimurium aroA strain caused lethal endotoxin shock to ddY mice. Treatment with PLB 1 h post infection (p.i.) resulted in significant reduction of mortality and bacterial numbers in livers. In addition, treatment with PLB 1 h p.i. resulted in a transient increase at the early stage and gradual decline in plasma LPS levels. Although plasma levels of sCD14 and high mobility group box chromosomal protein-1 (HMGB-1) increased according with progression of infection, increases in plasma levels of sCD14 and HMGB-1 were downregulated by treatment with PLB 1 h p.i. However, the lethal shock was not blocked by treatment with anti-CD14 monoclonal antibody at 3 h and 6 h p.i. Interestingly, administration of PLB 6 h p.i. did not show any protective activities, indicating that a time window for effective PLB action is present.
关键词：Salmonella;endotoxin;shock;polymyxin B;CD14;high mobility group box chromosomal protein-1 (HMGB-1)