文章基本信息

标题：Liver Site-Specific Gene Transfer Following the Administration of Naked Plasmid DNA to the Liver Surface in Mice
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作者：Ryu Hirayama ; Koyo Nishida ; Shintaro Fumoto 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：10
页码：1697-1699
DOI：10.1248/bpb.27.1697
出版社：The Pharmaceutical Society of Japan
摘要：The present study was undertaken to investigate liver site-specific gene transfer following the administration of naked plasmid DNA (pDNA) to the liver surface in mice. We examined whether genes could be delivered to the liver site specifically by utilizing the glass-made diffusion cell that is able to limit the contact dimension between the liver surface and pDNA solution administered. Gene expression was detected at the site of diffusion cell attachment (site 1) and was significantly higher than in other liver sites and tissues. Moreover, gene expression was also detected at deeper site from the liver surface (noncontact side with pDNA solution). The level of gene expression at site 1 did not change significantly with pDNA treatment for 10, 30, and 60 min. In conclusion, we demonstrated that naked pDNA administered to the liver surface in mice was taken up from its surface, and subsequently the protein encoded by pDNA could be produced site specifically.
关键词：gene therapy;liver;plasmid DNA;transfection;mice;luciferase