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标题：Secretion Mode and Subcellular Localization of Human Interferon-β Exogenously Expressed in Porcine Renal Epithelial LLC-PK1 Cells
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作者：Teruko Nishio ; Masato Maruyama ; Toyokazu Yoshida 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：10
页码：1653-1655
DOI：10.1248/bpb.27.1653
出版社：The Pharmaceutical Society of Japan
摘要：We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK₁. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans -Golgi network in a cell type-dependent manner.
关键词：epithelial cell;interferon;LLC-PK1;μ1B;polarity;secretory protein