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标题：Carbachol-Induced Membrane Ruffling and Its Desensitization in Rat Basophilic Leukemia (RBL-2H3) Cells Transfected with m3 Muscarinic Acetylcholine Receptors
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作者：Kazuhiko Oishi ; Tomokazu Yoshizumi ; Noriyasu Seki 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：10
页码：1544-1548
DOI：10.1248/bpb.27.1544
出版社：The Pharmaceutical Society of Japan
摘要：The changes in the reorganization of actin filaments during desensitization of secretion were investigated by transfecting RBL-2H3 cells with cDNA encoding the human m3 muscarinic acetylcholine receptors (RBL-m3 cells). Incubation of RBL-m3 cells with 10—100 μ M carbachol in Ca²⁺-free medium developed membrane ruffling. When the cells were desensitized under the condition where desensitization of carbachol-induced secretion occurred, desensitized cells failed to develop membrane ruffling with the subsequent addition of carbachol. These results suggest that m3 muscarinic receptor-mediated desensitization of secretion involves negative regulation of actin reorganization leading to membrane ruffling.
关键词：desensitization;muscarinic m3 receptor;RBL-2H3 cell;membrane ruffling;secretion