文章基本信息

标题：Convenient Procedures for Human Hair Protein Films and Properties of Alkaline Phosphatase Incorporated in the Film
本地全文：下载
作者：Toshihiro Fujii ; Daisuke Ogiwara ; Makoto Arimoto 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：1
页码：89-93
DOI：10.1248/bpb.27.89
出版社：The Pharmaceutical Society of Japan
摘要：We have developed a novel method of extracting proteins from human hair in the absence of detergent called the ‘Shindai Method’. Using the protein solution consisting of hard α-keratins and matrix proteins prepared by this method, we developed two procedures for preparing hair protein films. The protein solution was mixed with trichloroacetic acid (TCA), perchloric acid (PCA) or guanidine–HCl (GHA), and then exposed in distilled water. Light brown aggregates immediately formed (Pre-cast method). The other method is based on the same characteristics of the hair proteins to form protein aggregates. The protein was directly exposed to the solution containing TCA, PCA, GHA, HCl, H₂SO₄ or acetate buffer (Post-cast method). The maximum yield was greater than 70%. These protein films were water-insoluble and mainly made up of α-keratins. Scanning electron micrographs showed that the fine surface of the protein films was composed of particles, filaments, and porous structures and the constitution was dependent on the preparation procedure used. When porcine intestine alkaline phosphatase (ALP) was mixed with the hair protein solution in a Post-cast method using acetate buffer (pH 5), ALP was incorporated into the α-keratin films. The activity retained in the protein film was approximately 8% of the original level. The biochemical properties of the ALP activity in the film were similar to those of the native enzyme.
关键词：human hair protein;protein film preparation;keratin;alkaline phosphatase incorporation