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标题：Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents
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作者：Pramote Mahakunakorn ; Michihisa Tohda ; Yukihisa Murakami 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：1
页码：38-46
DOI：10.1248/bpb.27.38
出版社：The Pharmaceutical Society of Japan
摘要：The antioxidant properties of Choto-san and its related constituents such as Chotoko and Choto-san without Chotoko, and phenolic compounds contained in Chotoko such as epicatechin, caffeic, acid and quercetin were evaluated. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, the scavenging activity of Chotoko (IC₅₀ 14.3 μg/ml) was found to be higher than that of Choto-san (IC₅₀ 206.2 μg/ml) and Choto-san without Chotoko (IC₅₀ 244.3 μg/ml). Epicatechin (IC₅₀ 10.4 μ M ), caffeic acid (IC₅₀ 13.8 μ M ), and quercetin (IC₅₀ 7.1 μ M ) also revealed scavenging activity against DPPH radicals. Choto-san (IC₅₀ 67.7 μg/ml) exhibited stronger inhibitory activity against superoxide anion formation than Choto-san without Chotoko (IC₅₀ 92.4 μg/ml) but weaker activity than Chotoko (IC₅₀ 18.3 μg/ml). The generation of superoxide anion was also inhibited by epicatechin (IC₅₀ 175.2 μ M ), caffeic acid (IC₅₀ 141.7 μ M ), and quercetin (IC₅₀ 18.7 μ M ). In a hydroxyl radical-scavenging experiment, Choto-san (IC₅₀ 2.4 mg/ml), Chotoko (IC₅₀ 2.2 mg/ml), Choto-san without Chotoko (IC₅₀ 2.8 mg/ml), epicatechin (IC₅₀ 3.9 m M ), caffeic acid (IC₅₀ 3.6 m M ), and quercetin (IC₅₀ 1.9 m M ) exhibited activity. In NG108-15 cells, when added simultaneously with H₂O₂ (500 μ M ), Choto-san (250 μg/ml), Chotoko (250 μg/ml), Choto-san without Chotoko (500 μg/ml), epicatechin (200 μ M ), caffeic acid (200 μ M ), and quercetin (200 μ M ) effectively protected cells from oxidative damage. In conclusion, the present results provide evidence that Choto-san acts as an antioxidant and cytoprotective agent against oxidative damage, which is due at least partly to the phenolic compounds contained in Chotoko.
关键词：Choto-san;Chotoko;oxidative stress;free radical;antioxidant;NG108-15 cell