文章基本信息

标题：Effects of Oxygen on the Reactivity of Nitrogen Oxide Species Including Peroxynitrite
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作者：Kiyomi Kikugawa ; Kazuyuki Hiramoto ; Takumi Ohkawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2004
卷号：27
期号：1
页码：17-23
DOI：10.1248/bpb.27.17
出版社：The Pharmaceutical Society of Japan
摘要：This paper describes the O₂-dependent control of the reactivity of nitrogen oxide species for the production of biologically important nitrated and nitrosated compounds. In this study, the effects of O₂ on the reactivity of NO, NO₂, and ONOO⁻/ONOOH for nitration of tyrosine (Tyr) and nitrosation of glutathione (GSH) and morpholine (MOR) were examined. NO produced S -nitrosoglutathione (GSNO) and N -nitrosomorpholine (NMOR) through the formation of N₂O₃ under aerobic conditions, and NO₂ produced 3-nitrotyrosine (3-NO₂Tyr), GSNO, and NMOR. Transnitrosation from GSNO to MOR was observed only in the presence of O₂. Although preformed ONOO⁻/ONOOH produced all the products under aerobic conditions, the formation of 3-NO₂Tyr and GSNO was markedly reduced and the formation of NMOR was enhanced under anaerobic conditions. The reactivity of the CO₂ adduct of ONOO⁻ was similarly dependent on O₂. 3-NO₂Tyr was produced effectively by reaction with ONOO⁻/ONOOH at the O₂ concentration of 270 μ M and by reaction with its CO₂ adduct at O₂ concentrations greater than 5 μ M . Generation of ·OH from ONOO⁻/ONOOH was suppressed under anaerobic conditions. The reactivity of ONOO⁻/ONOOH and ·OH generation from ONOO⁻ were reversibly controlled by the O₂ concentration.
关键词：nitric oxide;peroxynitrite;tyrosine;glutathione;morpholine;oxygen