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标题：pH-Dependent Long-term Radical Scavenging Activity of AA-2G and 6-Octa-AA-2G against 2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Radical Cation
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作者：Jun Takebayashi ; Akihiro Tai ; Itaru Yamamoto 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：9
页码：1368-1370
DOI：10.1248/bpb.26.1368
出版社：The Pharmaceutical Society of Japan
摘要：The 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS^·+) decolorization assay was applied to evaluate the stoichiometric radical scavenging activity of ascorbic acid (AA) and two AA derivatives, 2- O -α- D -glucopyranosyl- L -ascorbic acid (AA-2G) and 2- O -α- D -glucopyranosyl-6- O -octanoyl- L -ascorbic acid (6-Octa-AA-2G). AA rapidly reacted with ABTS^·+, and the reaction was completed within 10 min. In contrast, AA-2G and 6-Octa-AA-2G continuously reacted with ABTS^·+, and the reaction was not completed after 2 h. The radical scavenging activity of AA-2G and 6-Octa-AA-2G in aqueous solutions at pH 4.0 and above was higher than that at pH 3.0, whereas AA showed no difference in the pH range 3 to 6. The amounts of ABTS^·+ scavenged by one molecule of AA, AA-2G and 6-Octa-AA-2G after 2 h of reaction at pH 6.0 were approximately 2.0, 3.4 or 3.9 molecules, respectively. This study demonstrates that the quantity of ABTS^·+ quenched by AA-2G and 6-Octa-AA-2G is superior to that of AA in a long-term reaction.
关键词：ascorbic acid 2-glucoside;6-acyl ascorbic acid 2-glucoside;radical scavenging;2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation