文章基本信息

标题：Inhibitory Effect of Green Tea Polyphenols on Membrane-Type 1 Matrix Metalloproteinase, MT1-MMP
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作者：Naoto Oku ; Motomi Matsukawa ; Satoru Yamakawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：9
页码：1235-1238
DOI：10.1248/bpb.26.1235
出版社：The Pharmaceutical Society of Japan
摘要：Matrix metalloproteinases (MMPs), especially membrane-type 1 matrix metalloproteinase (MT1-MMP), which generates an active form of MMP-2 from proMMP-2, are deeply involved in angiogenesis as well as in tumor cell migration and metastasis. To obtain a specific inhibitor for MT1-MMP, we screened a number of natural and synthetic compounds using recombinant human MMP-2, MMP-7, and soluble MT1-MMP in a fluorogenic peptide cleavage assay. (−)-Epigallocatechin 3- O -gallate (EGCG) followed by (−)-epigallocatechin 3,5-di- O -gallate and epitheaflagallin 3- O -gallate, was found to have potent and distinct inhibitory activity against MT1-MMP. Therefore, we investigated the effect of EGCG on the suppression of MMP-2 activation as determined by gelatin zymography, and observed that the active form of MMP-2 in the conditioned medium of human umbilical vein endothelial cells was decreased in the presence of EGCG. The results suggest the possibility that tea polyphenols suppress tumor growth through the suppression of angiogenesis.
关键词：membrane-type 1 matrix metalloproteinase (MT1-MMP);green tea;catechin;angiogenesis