文章基本信息

标题：Virus Concentration Using Sulfonated Magnetic Beads to Improve Sensitivity in Nucleic Acid Amplification Tests
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作者：Akiko Iwata ; Koei Satoh ; Mitsuhiro Murata 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：8
页码：1065-1069
DOI：10.1248/bpb.26.1065
出版社：The Pharmaceutical Society of Japan
摘要：To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration method using sulfonated (SO-) magnetic beads in the presence of divalent cations. In the presence of either Zn²⁺ or Cu²⁺ ions, we showed that SO-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine parvovirus (PPV) and poliovirus, which were not concentrated by polyethyleneimine (PEI)-magnetic beads.¹⁾ Using the SO-magnetic beads, the sensitivity of virus genome detection by PCR or RT-PCR can be enhanced. Therefore, an efficient virus concentration method using either SO-magnetic beads or PEI-magnetic beads enhances the sensitivity of virus detection by PCR or RT-PCR.
关键词：polymerase chain reaction (PCR);virus;divalent cation;nucleotide amplification tests (NAT)