文章基本信息

标题：Increased Expression of Hsp70 for Resistance to Deuterium Oxide in a Yeast Mutant Cell Line
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作者：Keiko Unno ; Takahiro Kishido ; Mizue Morioka 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：6
页码：799-802
DOI：10.1248/bpb.26.799
出版社：The Pharmaceutical Society of Japan
摘要：Labeling with stable isotopes, typically deuterium (D), is powerful tool for studying the functional structure of biomolecules by NMR. Biosynthesis of certain deuterated proteins in microorganisms cultured in deuterium oxide (D₂O) is an attractive strategy. However, the growth of almost all microorganisms is inhibited at high concentrations of D₂O. We isolated a mutant of yeast that grows well in D₂O. The expression of Hsp70 was enhanced in the mutant. The increased expression also endowed the yeast with cold-resistance. The mutant might be useful for biosynthesis of D-labeled biomolecules.
关键词：Hsp70;deuterium oxide (D2O);yeast;deuterium labeling;molecular chaperone