文章基本信息

标题：Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma
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作者：Naoko Yoshino ; Tetsuko Kojima ; Satoru Asami 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：5
页码：585-588
DOI：10.1248/bpb.26.585
出版社：The Pharmaceutical Society of Japan
摘要：Ewing's sarcoma (ES) is one of the most malignant bone and soft tissue tumors in childhood. Morphologically, ES belongs to the small round cell tumors (SRCT). ES, peripheral primitive neuroectodermal tumor (PNET), and Askin's tumor are classified as ES family tumors (ESFT) because they share a common chromosomal translocation. The EWS-FLI1 chimeric gene is generated by t (11; 22). Other reciprocal translocations resulting in formation of chimeric genes between EWS and ETS family genes ( ERG , ETV1 , E1AF , and FEV ) are t (21; 22), t (7; 22), t (17; 22), and t (2; 22), respectively. Although it is generally difficult to distinguish ES from SRCT, we could easily and quickly distinguish ES from other SRCT by using reverse transcription polymerase chain reaction (RT-PCR). We looked for specific chimeric genes in 23 tumor samples, including three ES clinical samples. We detected five chimeric genes in the three ES samples. Three chimeric genes, all EWS-FLI1 , were detected in one ES sample. Different chimeric genes, EWS-ERG and EWS-ETV1 , were detected in the other two ES samples. Moreover, because we could not detect specific chimeric genes in samples from non-ESFT, it may be possible to use this technique to diagnose ESFT and to detect tumor cell contamination before hematopoietic stem cell transplantation.
关键词：Ewing's sarcoma;chimeric gene;reverse transcription polymerase chain reaction