文章基本信息

标题：Rapid Determination of Total Bromide in Human Serum Using an Energy-Dispersive X-Ray Spectrometer
本地全文：下载
作者：Hiroko Tanaka ; Mariko Nakajima ; Manami Fujisawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：4
页码：457-461
DOI：10.1248/bpb.26.457
出版社：The Pharmaceutical Society of Japan
摘要：Since the bromide preparations useful in the treatment of intractable infantile epilepsy show a tendency to accumulate in the body, they may cause chronic toxicosis. To prevent this, determination of the bromide ion concentration in the serum is essential. After establishing a simple and rapid technique using energy-dispersive X-ray spectroscopy for the analysis of the serum total bromide level, we applied this technique in a clinically diagnosed epilepsy patient. The standard curve for total bromide showed linearity ( r =0.999) in the range of 10—2000 μg/ml, and the lowest detection limit was 5 μg/ml. The mean recovery rate of bromide added to reference serum to yield a concentration of 50 μg/ml was 93.5% ( n =5, coefficient of variation=9.1%). Analysis took only 20 min. On analysis of the serum of a 10-year-old girl whose treatment was initiated with orally administered potassium bromide 1.0 g/kg, a good correlation was found between the total bromide level obtained with energy-dispersive X-ray spectroscopic analysis and the level of bromide ions determined by ion-exchange HPLC. The determination of serum total bromide by rapid energy-dispersive X-ray spectroscopic analysis is a useful method of monitoring to prevent bromide poisoning.
关键词：bromide;energy-dispersive X-ray spectroscopy;epilepsy;serum;chronic toxicosis