文章基本信息

标题：Effects of Inorganic Anions on the Activation of Acid Sialidases
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作者：Megumi Nagaoka ; Takayuki Shiraishi ; Kimio Furuhata 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：3
页码：295-298
DOI：10.1248/bpb.26.295
出版社：The Pharmaceutical Society of Japan
摘要：An acid sialidase partially purified from porcine liver was activated by incubation at 37 °C under acidic pH. This activation was dependent on pH, time and temperature, but not inhibited by amastatin, an inhibitor of aminopeptidase A, in contrast to the case of human placental sialidase. The effects of inorganic anions on the two sialidases from porcine liver and from human placenta were investigated. Among the anions tested, halide ions, especially chloride and bromide ions, markedly enhanced the activation of the two sialidases. However, nitrate, sulfate, sulfite and pyrosulfite ions rarely affected the activation of sialidase from porcine liver, while all of them enhanced the activation of human placental sialidase. The activation of the enzyme from porcine liver was depressed at concentrations of greater than 100 m M of sodium chloride, whereas the enzyme from human placenta was held at maximum activation until 1 M sodium chloride. These results suggest the possibility of the participation of enzyme functions different from that of human placental sialidase in the activation process of sialidase.
关键词：sialidase;neuraminidase;inorganic anion effect