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标题：Molecular Cloning of the Gene for Edeine B1 Amidinohydrolase in Addition to the Agmatinase Activity in Bacillus subtilis
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作者：Kumiko Watanabe Shimotohno ; Tomomi Hidaka ; Takehiko Morishita 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：2
页码：262-265
DOI：10.1248/bpb.26.262
出版社：The Pharmaceutical Society of Japan
摘要：A gene with a high-nucleotide sequence homology to the edeine B₁ amidinohydrolase gene of Bacillus brevis was identified in the database of the Bacillus subtilis genome. The gene was isolated, expressed in Escherichia coli , and the gene product was analyzed with regard to the characteristics of its enzyme activity. A 32-kDa protein encoded by the ywhG gene showed a 69.8% amino acid sequence-homology to the edeine B₁ amidinohydrolase of B. brevis . Among various guanidino-compounds, edeine B₁ and agmatine were both efficiently hydrolyzed by the protein encoded by the ywhG gene, although edeine B₁ was a more potent substrate than agmatine in this assay system. These data indicate that the protein encoded by the ywhG gene is an agmatinase that is essential for polyamine biosynthesis in B. subtilis .
关键词：edeine B1 amidinohydrolase;agmatinase (agmatine ureohydrolase);ywhG (spe B);Bacillus subtilis;Bacillus brevis