文章基本信息

标题：Pharmacokinetic Study of β-Methyldigoxin by Enzyme Immunoassay Using a Novel Specific Antiserum in Rats
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作者：Yasuhiko Higashi ; Naomi Watanabe ; Toshiyuki Sasaki 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：2
页码：247-251
DOI：10.1248/bpb.26.247
出版社：The Pharmaceutical Society of Japan
摘要：We previously showed that enzyme immunoassay (EIA) of β-methyldigoxin (MDx3) using anti-MDx3 3′-hemisuccinate–bovine serum albumin antiserum (Antiserum-I) was superior to that using commercial anti-digoxin antiserum (Antiserum-II) in terms of specificity and that pretreatment of human serum with phenyl boric acid (PBA) column was effective. In the present study, we examined the precision of EIA using Antiserum-I and the recovery of MDx3 after PBA column treatment in rat serum, and also investigated pharmacokinetic changes of MDx3 in rats. The intra- and inter-assay variations and recovery tests using Antiserum-I were good. The PBA column was effective in selectively separating MDx3 from rat serum containing MDx3 and its metabolites. The recovery tests using Antiserum-I with PBA column showed about 110% and the interference of metabolites of MDx3 was negligible. Serum concentration–time courses of MDx3 by EIA using Antiserum-I with PBA column and Antiserum-I were lower than that using Antiserum-II. The distribution volume at steady state and total body clearance values of MDx3 in these conditions were significantly higher than those using Antiserum-II. The usefulness of PBA column was ascertained, while effects of PBA column on these parameters were not significant. In addition, rapid absorption of MDx3 was observed by EIA using Antiserum-I with PBA column. These results suggest that EIA using Antiserum-I with PBA column for the pretreatment of serum samples should be a more useful and valuable system in therapeutic drug monitoring and pharmacokinetic studies of the unchanged type of MDx3 than Antiserum-II.
关键词：enzyme immunoassay;β-methyldigoxin;pharmacokinetic study;anti-β-methyldigoxin 3′-hemisuccinate–bovine serum albumin antiserum;commercial anti-digoxin antiserum;phenyl boric acid column