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标题：Protective Effect of Aqueous Extract of Ginseng radix against 1-Methyl-4-phenylpyridinium-Induced Apoptosis in PC12 Cells
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作者：Ee-Hwa Kim ; Mi-Hyeon Jang ; Min-Chul Shin 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2003
卷号：26
期号：12
页码：1668-1673
DOI：10.1248/bpb.26.1668
出版社：The Pharmaceutical Society of Japan
摘要：Ginseng radix , the root of Panax ginseng C. A. M EYER (Araliaceae), is one of the best-known Oriental medicinal herbs with numerous therapeutic applications. To investigate whether Ginseng radix possesses a protective effect against 1-methyl-4-phenylpyridine (MPP⁺)-induced cytotoxicity in neuronal cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and caspase-3 enzyme assay were performed on PC12 neuronal cells. Cells treated with MPP⁺ exhibited various apoptotic features, while cell pretreated with Ginseng radix prior to MPP⁺ exposure showed a decrease in the occurrence of apoptotic features. These results suggest that Ginseng radix may exert a protective effect against MPP⁺-induced apoptosis in PC12 cells.
关键词：Ginseng radix;Parkinson's disease;1-methyl-4-phenylpyridine (MPP+);apoptosis