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标题：Antitumor-Promoting Constituents from Dioscorea bulbifera L. in JB6 Mouse Epidermal Cells
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作者：Huiyuan Gao ; Masanori Kuroyanagi ; Lijun Wu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2002
卷号：25
期号：9
页码：1241-1243
DOI：10.1248/bpb.25.1241
出版社：The Pharmaceutical Society of Japan
摘要：An antitumor-promoting effect was found in the extracts/ingredients of a plant used as a traditional medicine in mainland China, using the neoplastic transformation assay of mouse epidermal JB6 cell lines. The ethyl acetate soluble fraction of 75 % ethanol extract of the rhizomes of Dioscorea bulbifera L. showed an inhibitory effect against the tumor promotion of JB6 (Cl 22 and Cl 41) cells induced by a promoter, 12- O -tetradecanoylphorbol-13-acetate (TPA). Further investigation on the constituents of the EtOAc fraction from the rhizomes revealed the chemical structure to be kaempferol-3,5-dimethyl ether (1), caryatin (2), (+)-catechin (3), myricetin (4), quercetin-3- O -galactopyranoside (5), myricetin-3- O -galactopyranoside (6), myricetin-3- O -glucopyranoside (7) and diosbulbin B (8). Constituent antitumor-promoting activities were also examined in the same way. Compounds 1—7, characterized as flavonoids with the two hydroxyl groups at C-7 and C-4′, showed the most potent inhibitory effect, but there seemed to be differences in the inhibitory effect between flavonol aglycones and flavonol glycosides. Compared with (−)-epicatechin, (+)-catechin exhibited much stronger inhibitory activity which suggested that chemical stereo structures of compounds affect the efficiency of inhibition. Compound 8 showed moderate activity. The constituents with antitumor-promoting activity from this plant are reported for the first time.
关键词：antitumor-promoting activity;JB6 cell;soft agar colony formation method;Dioscorea bulbifera L.;flavonoid glycoside